This implies that X-ray phase-contrast imaging may also lead to the development of entirely new (micro-) radiological applications. This review provides a brief overview of the physical characteristics of this new technology and describes recent developments towards clinical implementation of X-ray phase-contrast imaging of the breast. Also dose measurements were performed. The first imaging results obtained with the system show the complementary nature of phase-contrast micro-CT images with respect to absorption-based micro-CT. Future improvements, necessary to optimize the scanner for in-vivo small-animal CT scanning on a regular and easy-to-use basis, are also discussed.
Phase contrast in laboratory-based X-ray CT Y. De Witte 1, M. N. Boone , B. Pauwels 2, E. Herremans3, E. Van de Casteele , L. Van Hoorebeke1 1 UGCT, Ghent University, Proeftuinstraat 86, 9000 Ghent, Belgium [email protected] 2 SkyScan, Kartuizersweg 3B, 2550 Kontich, Belgium 3 MeBioS, K.U.Leuven, Willem de Croylaan 42, 3001 Heverlee, Belgium When scanning weakly attenuating samples using ... Phase-contrast CT provided microscopic detail of soft-tissue anatomy in the mummified hand of an Egyptian boy who probably lived 2,400 years ago. The researchers believe the resolution of phase-contrast CT surpasses that of conventional CT by a factor of 100 -- and could someday be useful in the clinical realm. This technique provides a fast, nondestructive, and 3D quantification of bone scaffolds, bone ingrowth, and microvascularization. Synchrotron radiation absorption and phase micro-CT offer additional advantages to image newly formed bone in bioceramic scaffolds and pre-bone matrix.
X-ray micro-computed tomography (micro-CT) is used for in-vivo tumor imaging in small-animal models of human disease for the purpose of assessing the effectiveness of experimental drugs and/or treatments. We have been constructing, developing, as well as investigating the imaging performance experimentally, of an in-vivo , in-line holography, x-ray phase-contrast, cone-beam, micro-CT system ... X-ray absorption-contrast micro-computed tomography (CT) imaging and magnetic resonance imaging (MRI) have been used to image three-dimensional scaffolds in biological research studies. However, MRI often requires contrast enhancement and it is difﬁcult to visualize three-dimensional microstructure of scaffolds due to limitations in temporal and
One of the major drawbacks of CT and micro-CT is their rather poor density resolution (smallest density variation in the object that can be resolved in the image). In-line x-ray phase-contrast imaging is a promising new technology that might afford improved soft tissue resolution [1-11]. This imaging method requires an x-ray source with a ... Phase-contrast CT: Fundamental theorem and fast image reconstruction algorithms Andrei V. Bronnikov Bronnikov Algorithms, PO Box 77, Arnhem 6800 AB, The Netherlands ABSTRACT Phase-contrast x-ray computed tomography (CT) is an emerging imaging technique that can be implemented
Phase information has been inferred from images obtained at the same x-ray energy but at different object-to-detector distances and also from images obtained at the same object-to-detector distance but with different K-alpha line energies. Ultrafast laser-based, compact, x-ray source is a promising technique for micro-CT systems. micro-CT and previous phase-contrast methods, granting the possibility of performing longitudinal studies. We note that CO 2 is already used clinically as an x-ray contrast agent by injecting it into the blood stream to temporarily replace the blood and thereby reduce the x-ray absorption in the vessels (Cho and Hawkins 2007).
Phase-contrast X-ray imaging (PCI) or phase-sensitive X-ray imaging is a general term for different technical methods that use information concerning changes in the phase of an X-ray beam that passes through an object in order to create its images. Standard X-ray imaging techniques like radiography or computed tomography (CT) rely on a decrease of the X-ray beam's intensity (attenuation) when ... Belg. J. Zool., 144(1) : 20-40 January 2014 Soft tissue discrimination with contrast agents using micro-CT scanning Emilie Descamps 1, Alicja Sochacka 1, Barbara De Kegel 1, Denis Van Loo 2, 3 ...
Micro-CT (micro tomography) uses X-ray scanning to recreate a 3D model without destroying the original object. Micro computed tomography or “micro-CT” is X-ray imaging in 3D, by the same method used in hospital CT (or “CAT”) scans, but on a small scale with massively increased resolution. Another milestone is the implementation of grating-based phase-contrast CT equipment, including a rotating x-ray tube and grating interferometer, in a gantry-based system, which was recently realized in a first phase-contrast micro-CT small-animal scanner (34 – 36). This advancement has opened up the opportunity to examine higher numbers of ... Boone, Matthieu, Devulder, W., Dierick, M., & Van Hoorebeke, L. (2011). Application of phase contrast tomography in laboratory-based X-ray micro-CT. Digital Industrial Radiology and Computed Tomography, International symposium, Abstracts. Presented at the International symposium on Digital Industrial Radiology and Computed Tomography.
Phase-contrast micro-CT achieved cellu-lar resolution of chondrocytes and quantitative assess-ment of parameters such as articular cartilage volume and surface area. In mouse models of OA generated by either CLT or DMM, we showed that phase-contrast micro-CT distinguished control and OA cartilage by providing quantitative measures with high ... X-ray microtomography and segmentation of phase contrast images G. van Dalen 1, A. Don , P. Nootenboom1, M. van Ginkel2, E. Boller3, M. Langer3 1 Unilever R&D, Vlaardingen ,the Netherlands Gerard ...
Phase-contrast imaging is a method of imaging that has a range of different applications. It exploits differences in the refractive index of different materials to differentiate between structures under analysis. In conventional light microscopy, phase contrast can be employed to distinguish between structures of similar transparency, and to examine crystals on the basis of their double ... 1. Am J Physiol Lung Cell Mol Physiol. 2019 Oct 9. doi: 10.1152/ajplung.00103.2019. [Epub ahead of print] Synchrotron-based phase contrast micro-CT as a tool for understanding pulmonary vascular pathobiology and the 3-D micro-anatomy of alveolar capillary dysplasia. Three-Dimensional Imaging of Nerve Tissue by X-Ray Phase-Contrast Microtomography F. Beckmann,* K. Heise, #B. Ko¨lsch, U. Bonse,* M. F. Rajewsky, M. Bartscher,* and T. Biermann* *Institute of Physics, University of Dortmund, D-44221 Dortmund, Germany, and #Institute of Cell Biology (Cancer Research), University of Essen Medical School, D-45122 Essen, Germany
Phase contrast microscopy, first described in 1934 by Dutch physicist Frits Zernike, is a contrast-enhancing optical technique that can be utilized to produce high-contrast images of transparent specimens such as living cells, microorganisms, thin tissue slices, lithographic patterns, and sub-cellular particles (such as nuclei and other organelles). Its utilization might result in faster scans with lower radiation dose, better spatial and contrast resolution and also femtosecond temporal resolution. In addition, it might allow practical implementation of dual-energy and phase-contrast imaging micro-CT that is not possible with conventional micro-CT. In this study, a new in-line phase-contrast cone-beam CT (PC-CBCT) system has been designed and tested in our lab to produce higher image quality enhancement. The PC-CBCT system consists of a micro-focus x-ray tube, a high-resolution detector and a rotating phantom holder.
So you are getting a pseudo darkfield microscope as well as a phase contrast instrument! This will show your specimen, not as a phase image, but illuminated on a black background. View recommended suppliers to view phase contrast models and prices. Learn more in depth about phase contrast microscopy here. Background • Aim: Monte Carlo simulation of X-ray in-line phase contrast –Infrastructure for grid computing exist (VIP, Gatelab, …) –Focus is for the moment on the physics problems
based contrast. One example is propagation or in-line phase contrast, a refractive index-based source of contrast that comes from interference of x-rays in the object (Snigirev 1995et al). Propagation based phase contrast micro-CT has shown potential for imaging cortical micro- X-ray phase-contrast tomography for high-spatial-resolution zebrafish muscle imaging ... When working in synchrotron absorption micro-CT, ... J. et al. Phase-contrast X-ray CT Imaging of Esophagus ... This study aimed to explore the value of synchrotron-based phase-contrast microcomputed tomography (micro-CT) in pulmonary vascular pathobiology. The microanatomy of the lung is complex with intricate branching patterns. Tissue sections are therefore difficult to interpret. Recruited intrapulmonary bronchopulmonary anastomoses (IBAs) have been described in several forms of pulmonary ...
Correlative Light and Electron Microscopy (CLEM) is one way to combine functional and structural imaging on the same samples. For multicellular models, X-ray Computed Tomography (CT) has a crucial role to play in such combined workflows as it bridges the resolution gap and enables precise targeting of key features within the tissues. Application of phase contrast tomography in laboratory-based X-ray micro-CT . By Matthieu Boone, Wouter Devulder, Manuel Dierick and Luc Van Hoorebeke. Abstract. Driven by the technical and computational evolution, X-ray micro-CT (µCT) has become a wide-spread tool in 3D imaging and analysis. In recent years, refraction of the X-rays has ... AUGMENTING SOFT TISSUE CONTRAST USING PHASE-CONTRAST TECHNIQUES IN MICRO-COMPUTED TOMOGRAPHY IMAGING Aino Reunamo Master’s Thesis University of Eastern Finland Department of Applied Physics January 24, 2019
Specimens were imaged with micro-CT following two protocols: 1) absorption contrast (AC) imaging 2) propagation phase-contrast (PPC) imaging. All samples were scanned in liquid. The contrast to noise ratio (C/N) of cellular features quantified scan quality and were statistically analysed. ZEISS has now expanded its portfolio of lab X-ray imaging instruments with a new micro-computed tomography (microCT) system: ZEISS Xradia Context microCT. ... which are resolved with high contrast. ... or with advanced imaging modalities like phase contrast or diffraction contrast tomography. ...
Flexible solutions for lab-based phase contrast and dark field CT and micro-CT Alessandro Olivo1, Fabio A. Vittoria1, Peter Modregger1, Gibril K. Kallon1, Lorenzo Massimi1, Jinxing Jiang1, Savvas Savvidis1, Luca Brombal2, Glafkos Havariyoun1, Charlotte J. Maughan Jones1, Ian Buchanan1, Dana Shoukroun1, Peter R. T. Advanced X-ray computed tomography methods: High resolution CT, quantitative CT, 4DCT and phase contrast CT Johann KASTNER1, Bernhard PLANK1 and Christoph HEINZL1 1University of Applied Sciences Upper Austria, Stelzhamerstrasse 23, 4600 Wels, Austria Abstract During the last years, a number of novel X-ray imaging and data processing methods have been developed. Phase contrast X-ray micro CT analysis of the 3D structure of the developing teeth and head skeletal elements that are involved in feeding and respiration in the zebrafish and medaka fish Ä è sa (!° û$-b Å Ä!
To overcome the limitations of X-ray absorption micro-CT, several X-ray phase-contrast imaging techniques have been developed in the last decades 12,13. However, these methods often require a ... We compare visualization and contrast of conventional absorption-based micro-CT to synchrotron radiation phase contrast imaging (SR-PCI) images of whole unstained, nondecalcified human cochleae. Three cadaveric cochleae were imaged using SR-PCI and micro-CT. Geometry-constraint-scan imaging for in-line phase contrast micro-CT Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 3rd International Conference on Biomedical Engineering and Biotechnology, 25–28 September 2014, Beijing, China ...
SkyScan 1294 – Phase-Contrast Desk-Top X-Ray micro-CT Phase contrast microtomography allows us to non-destructively visualize previously inaccessible information inside an object in 3D. The SkyScan 1294 makes use of unique technology developed at and patented by the Paul Scherrer Institute in Switzerland, A phase-contrast X-ray microtomography system using the Talbot imaging has been built at the SPring-8 synchrotron radiation facility. This system has much higher density resolution than absorption-based X-ray microtomography. The tomographic sections of formalin-fixed mouse fetuses obtained with this method clearly depict various organs without any staining at a pixel resolution of up to 5 µm ... Overcome the limits of micro-CT solutions by faster 3D X-ray microscopy imaging of intact samples at sub-micron scale resolution ... Maximize absorption and phase contrast to achieve unprecedented characterisation of your materials and their properties. Unlock 3D crystallographic information with Diffraction Contrast Tomography.
In this work, we used phase-contrast assisted micro-CT with potassium iodine-doped brine to visualize four phases: sand particles, pore fluid, methane hydrate and methane gas. This study visualizes the pore habit of methane hydrate at various stages including during hydrate formation in excess-gas systems, its evolution after brine injection to ... of a biological specimen with complementary contrast in the at-tenuation and phase image. Results Design and Layout of the First Preclinical Phase-Contrast CT Scanner. The prototype scanner consists of a standard rotating micro-CT gantry (with X-ray source, specimen opening, and a flat-panel Industrial, micro- and nano-CT as well as 3D X-ray microscopes typically rotate the specimen through a stationary X-ray beam for this purpose. This is in contrast to hospital X-ray CT, where the X-ray source and ... Phase contrast11 is primarily used for imaging materials that have low absorption, or to differentiate materials of similar ...
phase contrast-micro-CT. Reprogramming Genes to Keep Joints Healthy. Posted on March 19th, 2013 by Dr. Francis Collins . Credit: Brendan Lee and Zhechao Ruan, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX. phase shift, such as grating-based phase retrieval ,  and multiple-image holotomography , , where phase shift is visualized by in-line beam propagation. Similarly, beam propagation also creates phase contrast in standard micro-CT, where projections from multiple angles are used to create a three dimensional image of the object.
In this study, we compare the visualization and contrast capabilities of two high resolution imaging modalities: conventional absorption‐based micro‐CT and synchrotron radiation phase contrast imaging (SR‐PCI). We compare the two modalities using images of three unstained, nondecalcified cadaveric human cochleae. Phase contrast tomography in laboratory-based X-ray micro-CT Matthieu N. Boone*, Yoni De Witte, Manuel Dierick, Luc Van Hoorebeke UGCT, Dept. Physics and Astronomy, Institute for Nuclear Sciences, Proeftuinstraat 86,
Phase Contrast Micro Ct © 2020 Phase contrast microscopy, first described in 1934 by Dutch physicist Frits Zernike, is a contrast-enhancing optical technique that can be utilized to produce high-co